LOCUS       sequence-67692-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses myc, HIS tagged EWSR1 in mammalian cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Pahlich S, Zakaryan RP, Gehring H
  TITLE     Identification of proteins interacting with protein arginine 
            methyltransferase 8: the Ewing sarcoma (EWS) protein binds 
            independent of its methylation state.
  JOURNAL   Proteins. 2008 Sep;72(4):1125-37. doi: 10.1002/prot.22004.
  PUBMED    18320585
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/prot"; 
            journalName: "Proteins"; date: "2008-09"; volume: "72"; issue: "4"; 
            pages: "1125-37"
COMMENT     Sequence Label: pcDNA3.1 EWS-myc-HIS
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..29)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(45..74)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cgtttaactc aatgatgatg atgatgatgg tcgacggcgc tattcagatc ctcttctgag
       61 atgagttttt gttctagaaa gcttggtacc gagctcggat ccactagtcc agtgtggtgg
      121 aattcgtagg gccgatctct gcgctcctga cggtgctcgc ctttatccat ttttccaggt
      181 cctccacgtc ctcctcttct tcctcccatc tgttccatca aaggtccagg gggcccccca
      241 gggccacctc gtcttcctcc accaaagcca cctcggtcca tgccccggcc accacggaag
      301 ccacctctgt ctccaccacg gccacctctg aacattccac cgggaccacc acgatccatg
      361 aggccacctc ttcctccccg catgccacca gggccacctc tgccacgatc accacccggg
      421 ggcggaaagg gtggcgggag gaagccttca ggctttgggg ccttacactg gttgcactct
      481 gttctccagg cgaagttctg gtttccacaa cccggattgg gacactgcca gtctccagct
      541 cggtgctgga cgtttcctcc tccagagggg ttccctcggg aaccccgggg tcctcttgga
      601 gggaagcctc ctctatctcc tccacggcct cccatgcgac ccatgggtcc cccaggacct
      661 cctgggcctc ctggacctcc acggagtggt ggtggcatgc ctctgccctc acggggtggc
      721 agaccacccc gcatactgtt cattggaggc ttcttccgag caagggagac tttaagtttg
      781 ctcccttgaa aatctttccc atcaaaccat tccacggcag ccttggcagt gggtggg
//
