LOCUS       sequence-67704-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses myc, HIS tagged EWSR1 in mammalian cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Pahlich S, Zakaryan RP, Gehring H
  TITLE     Identification of proteins interacting with protein arginine 
            methyltransferase 8: the Ewing sarcoma (EWS) protein binds 
            independent of its methylation state.
  JOURNAL   Proteins. 2008 Sep;72(4):1125-37. doi: 10.1002/prot.22004.
  PUBMED    18320585
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/prot"; 
            journalName: "Proteins"; date: "2008-09"; volume: "72"; issue: "4"; 
            pages: "1125-37"
COMMENT     Sequence Label: pcDNA3.1 EWS-myc-HIS
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      107..116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cgggccctct agactcgagc ggccgcgcca ccatggcgtc
      121 cacggattac agtacctata gccaagctgc agcgcagcag ggctacagtg cttacaccgc
      181 ccagcccact caaggatatg cacagaccac ccaggcatat gggcaacaaa gctatggaac
      241 ctatggacag cccactgatg tcagctatac ccaggctcag accactgcaa cctatgggca
      301 gaccgcctat gcaacttctt atggacagcc tcccactggt tatactactc caactgcccc
      361 ccaggcatac agccagcctg tccaggggta tggcactggt gcttatgata ccaccactgc
      421 tacagtcacc accacccagg cctcctatgc agctcagtct gcatatggca ctcagcctgc
      481 ttatccagcc tatgggcagc agccagcagc cactgcacct acaagaccgc aggatggaaa
      541 caagcccact gagactagtc aacctcaatc tagcacaggg ggttacaacc agcccagcct
      601 aggatatgga cagagtaact acagttatcc ccaggtacct gggagctacc ccatgcagcc
      661 agtcactgca cctccatcct accctcctac cagctattcc tctacacagc cgactagtta
      721 tgatcagagc agttactctc agcagaacac ctatgggcaa ccgagcagct atggacagca
      781 gagtagctat ggtcaacaaa gcagctatgg gcagcagcct cc
//
