LOCUS       sequence-67705-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Hocine S, Raymond P, Zenklusen D, Chao JA, Singer RH
  TITLE     Single-molecule analysis of gene expression using two-color RNA 
            labeling in live yeast.
  JOURNAL   Nat Methods. 2013 Feb;10(2):119-21. doi: 10.1038/nmeth.2305. Epub 
            2012 Dec 23.
  PUBMED    23263691
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2305"; journalName: "Nat Methods"; date: "2013-02"; 
            volume: "10"; issue: "2"; pages: "119-21"
COMMENT     Sequence Label: pDZ416 (24PP7SL loxP-Kan-loxP)
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(36..379)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    complement(434..467)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgcggcctcg aaacgtgagt cttttcctta cccatggttg tttatgttcg gatgtgatgt
       61 gagaactgta tcctagcaag attttaaaag gaagtatatg aaagaagaac ctcagtggca
      121 aatcctaacc ttttatattt ctctacaggg gcgcggcgtg gggacaattc aacgcgtctg
      181 tgaggggagc gtttccctgc tcgcaggtct gcagcgagga gccgtaattt ttgcttcgcg
      241 ccgtgcggcc atcaaaatgt atggatgcaa atgattatac atggggatgt atgggctaaa
      301 tgtacgggcg acagtcacat catgcccctg agctgcgcac gtcaagactg tcaaggaggg
      361 tattctgggc ctccatgtcg ctggccgggt gacccggcgg ggacgaggca agctaaacag
      421 atctctagac ctaataactt cgtatagcat acattatacg aagttatatt aagggttgtc
      481 gacctgcagc gtacaattcc tgcagcccgg gggatctaat gaacccggga atactgcagc
      541 cagcgagccc atatgctctg ctggtttcta gagtcgacct gcagggagcg acgccatatc
      601 gtctgctcct ttctaggcaa ttaggtacct taggatctaa tgaacccggg aatactgcag
      661 ccagcgagcc catatgctct gctggtttct agagtcgacc tgcagggagc gacgccatat
      721 cgtctgctcc tttctaggca attaggtacc ttaggatcta atgaacccgg gaatactgca
      781 gccagcgagc ccatatgctc tgctggtttc tagagtcgac ctgcagggag cg
//
