LOCUS       sequence-67710-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Hocine S, Raymond P, Zenklusen D, Chao JA, Singer RH
  TITLE     Single-molecule analysis of gene expression using two-color RNA 
            labeling in live yeast.
  JOURNAL   Nat Methods. 2013 Feb;10(2):119-21. doi: 10.1038/nmeth.2305. Epub 
            2012 Dec 23.
  PUBMED    23263691
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2305"; journalName: "Nat Methods"; date: "2013-02"; 
            volume: "10"; issue: "2"; pages: "119-21"
COMMENT     Sequence Label: pSH100 (YCplac33 MET25pro MCP-mCherry)
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(32..279)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     245..263
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     281..297
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     282..298
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(579..598)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     848..866
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gactcactat agggcgaatt gggtaccggc cgcaaattaa agccttcgag cgtcccaaaa
       61 ccttctcaag caaggttttc agtataatgt tacatgcgta cacgcgtctg tacagaaaaa
      121 aaagaaaaat ttgaaatata aataacgttc ttaatactaa cataactata aaaaaataaa
      181 tagggaccta gacttcaggt tgtctaactc cttccttttc ggttagagcg gatgtggggg
      241 gagggcgtga atgtaagcgt gacataacta attacatgac tcgaggtcga cggtatcgat
      301 ttacttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg
      361 ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt tgacgttgta
      421 ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cagcgtcgta
      481 gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg cgccgtcctc
      541 ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca ttacggggcc
      601 gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc cgtcctgcag
      661 ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca
      721 cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac
      781 gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga agggcagggg
      841 gccacccttg gtcaccttca gcttggcggt ctggtgctct agg
//
