LOCUS       sequence-67719-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Ren X, Hurley JH
  TITLE     Structural basis for endosomal recruitment of ESCRT-I by ESCRT-0 in 
            yeast.
  JOURNAL   EMBO J. 2011 Jun 1;30(11):2130-9. doi: 10.1038/emboj.2011.122. Epub 
            2011 Apr 19.
  PUBMED    21505419
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2011.122"; journalName: "EMBO J"; date: "2011-06-1- 
            1"; volume: "30"; issue: "11"; pages: "2130-9"
COMMENT     Sequence Label: YCplac111-yVps23-mCherry
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     256..278
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     270..287
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     271..287
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(874..893)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgatattagg acttccacac caactagtaa tatgacaata aattcaagat attcttcatg
       61 agaatggccc agcgattatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
      121 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
      181 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
      241 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttcggagtgt
      301 ctagtgtggg caagggaggt cttgttgcgt gtctgtgcat ctgcttgtac gccaacgcgg
      361 aggtcgacga ggaactgcaa cgcaccatgg tagcacatga agaagcaacc accctgcggt
      421 ggccgctttg caataacgat ctcagcatta gaaagactgg gttagtgagc tcagatgttc
      481 taaagattct gtatatacga actatgtact tccatatata ccattccccg ttcttgttag
      541 gtaaaggtta aaaaaatatt ttttatggca cttcggcgat gcgaaagaaa gtgagtcact
      601 tgtacagctc gtccatgccg ccagtggagt ggcggccctc ggcgcgttcg tactgttcca
      661 cgatggtgta gtcctcgttg tgggaggtga tgtccaactt gatgttgacg ttgtaggcgc
      721 cgggcagctg cacgggcttc ttggccttgt aggtggtctt gacctcggcg tcgtagtggc
      781 cgccgtcctt cagcttcagc ctctgcttga tctcgccctt cagggcgccg tcctcggggt
      841 acatccgctc ggaggaggcc tcccagccca tggtcttctt ctgcattacg gggccgtcgg
      901 aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa ctcgccgtcc tgcagggagg
      961 a
//
