LOCUS       sequence-67732-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Friedman LJ, Gelles J
  TITLE     Mechanism of transcription initiation at an activator-dependent 
            promoter defined by single-molecule observation.
  JOURNAL   Cell. 2012 Feb 17;148(4):679-89. doi: 10.1016/j.cell.2012.01.018.
  PUBMED    22341441
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.01"; journalName: "Cell"; date: "2012-02-17- 
            17"; volume: "148"; issue: "4"; pages: "679-89"
COMMENT     Sequence Label: pNoProm-1
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(735..754)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(736..754)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(773..789)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(773..789)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(786..808)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    797..813
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(821..827,828..845,846..851))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    866..887
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gagctcggta cccggggatc gctttcacgg agcgttaaaa gggtggcttg agatcagttg
       61 cactaaaatg gtgcataatg ttaacattaa tgcactaaaa tggtgcaaca tgatcatatc
      121 gtcaattatt acctccacgg ggagagcctg agcaaactgg cctcaggcat ttgagaagca
      181 cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga cataagcggc
      241 tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga atttctgcca
      301 ttcatccgct tattatcact tattcaggcg tagcaaccag gcgtttaagg gcaccaataa
      361 ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
      421 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
      481 atcagcacct tgtcgccttg cgtataatat ttgcccatgg gaaagacggc gacacggcca
      541 gcagaaccaa agacaccaca gaccacacac aagacaccac agaccacaca caagacacca
      601 cagaccacac acaagacacc acagaccaca cacaagacac cacagaccac acacaagaca
      661 ccacagacca cacacaagac accacagacc acacacaaga cttggttttg tttacctgca
      721 ggcatgcaag ctttccctat agtgagtcgt attagagctt ggcgtaatca tggtcatagc
      781 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
      841 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
      901 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
//
