LOCUS       sequence-67745-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Carlson LA, Hurley JH
  TITLE     In vitro reconstitution of the ordered assembly of the endosomal 
            sorting complex required for transport at membrane-bound HIV-1 Gag 
            clusters.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Oct 16;109(42):16928-33. doi: 
            10.1073/pnas.1211759109. Epub 2012 Oct 1.
  PUBMED    23027949
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1211759109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-10-16- 16"; volume: "109"; issue: "42"; pages: 
            "16928-33"
COMMENT     Sequence Label: pHisAlix
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             62..79
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             101..121
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgtcgtacta
       61 ccatcaccat caccatcacg attacgatat cccaacgacc gaaaacctgt attttcaggg
      121 cgccatggga tccatggcga cattcatctc ggtgcagctg aaaaagacct cagaggtgga
      181 cctggccaag ccgctggtga agttcatcca gcagacttac ccaagcggcg gggaagagca
      241 ggcccagtac tgccgcgcgg cggaggagct cagcaagctg cgccgcgccg cagtcggtcg
      301 tccgctggac aagcacgagg gcgcgctcga gacgctcctg agatattatg atcagatttg
      361 ttctattgaa cccaaattcc cattttctga aaatcagatc tgcttgacat ttacctggaa
      421 ggatgctttc gataaaggtt cactttttgg aggctctgta aaactggctc ttgcaagctt
      481 aggatatgaa aagagctgtg tgttgttcaa ttgtgcagcc ttagctagcc aaattgcagc
      541 agaacagaac ctggataatg atgaaggatt gaaaatcgct gctaaacatt accagtttgc
      601 tagtggtgcc tttttacata ttaaagagac ggttttatct gccttaagtc gagagccgac
      661 cgtggacata tctccagata ctgttgggac cctcagtctt attatgctgg cacaggctca
      721 agaagtattt tttttaaaag ccacaagaga taaaatgaaa gatgccatca tagctaaatt
      781 ggctaatcag gctgcagatt attttggtga tgctttcaaa cagtgtcaat acaaagatac
      841 tctccccaag gaggtgttcc ctgtcttggc tgcaaagcac tgtatcatgc aggccaatgc
      901 tgagtaccat cagtctatcc tg
//
