LOCUS       sequence-67762-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Ren X, Hurley JH
  TITLE     VHS domains of ESCRT-0 cooperate in high-avidity binding to 
            polyubiquitinated cargo.
  JOURNAL   EMBO J. 2010 Mar 17;29(6):1045-54. Epub 2010 Feb 11.
  PUBMED    20150893
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2010-03-17"; volume: "29(6)"; pages: "1045-54. Epub 2010 Feb 
            11"
COMMENT     Sequence Label: pGST2-STAM1-VHS
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..45
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(549..571)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(716..734)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        802..906
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catcctccaa aaatcgatac gaccgaaaac ctgtattttc agggcgccat gggacctctt
       61 tttgccacca atcccttcga tcaggatgtt gagaaagcaa ccagcgagat gaatactgct
      121 gaggactggg gcctcatttt ggatatctgt gataaagttg gtcagtctcg cactggacct
      181 aaggattgtc ttcggtctat tatgagaaga gtgaaccaca aagatcctca cgttgctatg
      241 caggctttga ctcttctagg agcatgtgta tcaaactgtg gcaaaatttt tcatttagaa
      301 gtatgttcaa gagattttgc tagtgaagta agcaacgtat taaataaggg tcatcctaaa
      361 gtatgtgaaa aattaaaggc tcttatggtt gaatggacag atgaatttaa gaatgatcca
      421 cagcttagtc taatatcagc aatgattaag aacctcaagg aacaaggagt tacgttccca
      481 taactcgagc ggccgcatcg tgactgactg acgatctgcc tcgcgcgttt cggtgatgac
      541 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
      601 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
      661 gccatgaccc agtcacgtag cgatagcgga gtgtataatt cttgaagacg aaagggcctc
      721 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
      781 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
      841 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      901 aagagtatga gtattccaac atttccgtgt cgc
//
