LOCUS       sequence-67768-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Falaleeva M, Sulsona CR, Zielke HR, Currey KM, de la Grange P, 
            Aslanzadeh V, Driscoll DJ, Stamm S
  TITLE     Molecular characterization of a patient presumed to have 
            prader-willi syndrome.
  JOURNAL   Clin Med Insights Case Rep. 2013 May 5;6:79-86. doi: 
            10.4137/CCRep.S11510. Print 2013.
  PUBMED    23700380
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Clin Med 
            Insights Case Rep."; date: "2013-05-5"; volume: "6:79-86. doi"; 
            pages: " 10.4137/CCRep.S11510. Print 2013"
COMMENT     Sequence Label: pCRII-SNORD115-11
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(237..255)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(238..255)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(273..289)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(273..289)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(286..308)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    297..313
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(321..327,328..345,346..351))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    366..387
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(504..521)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(755..774)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatagggcga attgggccct ctagatgcat gctcgagcgg ccgccagtgt gatggatatc
       61 tgcagaattc gcccttggcc tcagcgtaat cctattgagc atgattttta agtcatcacc
      121 tctcttcagg acaatataag gttctcatca ttgacccaag ggcgaattcc agcacactgg
      181 cggccgttac tagtggatcc gagctcggta ccaagcttga tgcatagctt gagtattcta
      241 tagtgtcacc taaatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
      301 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      361 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
      421 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
      481 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      541 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      601 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      661 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      721 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      781 agctccctcg tgcgctc
//
