LOCUS       sequence-67769-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Ragusa MJ, Stanley RE, Hurley JH
  TITLE     Architecture of the Atg17 complex as a scaffold for autophagosome 
            biogenesis.
  JOURNAL   Cell. 2012 Dec 21;151(7):1501-12. doi: 10.1016/j.cell.2012.11.028. 
            Epub 2012 Dec 6.
  PUBMED    23219485
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.11.028"; journalName: "Cell"; date: 
            "2012-12-21- 21"; volume: "151"; issue: "7"; pages: "1501-12"
COMMENT     Sequence Label: pHIS2-KL EAT
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             62..79
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             101..121
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             736..747
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     RBS             854..862
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgtcgtacta
       61 ccatcaccat caccatcacg attacgatat cccaacgacc gaaaacctgt attttcaggg
      121 cgccatggga tccgattcca acataactcc tgcagtggaa tcgcttgcgg ccaaagcatt
      181 tgtcatgtat tcgttcgctg aaatgaaatt ctctcaaatt cttccaactc ctccttcttc
      241 aaccgactat gacccactgt cagataaacg tctaagtaac ggaagttgcg cgatagagga
      301 tgaagaagat ttggatcaag gccgcccacc ttctaatcaa actttgacct cagcaacaac
      361 aaagatttca tcagccacca atgttgacac tcaaataccg gcccccgagt tgaaaaagtt
      421 gtgcaccgaa tctttacttc tttatctaaa agctcttact attttagctg cttcaatgaa
      481 attaacatca aaatggtggt atgaaaatga aagtaaaaac tgcactttga agctaaacat
      541 tttggtgcag tggattagag atcgctttaa cgaatgctta gacaaggctg aatttttgcg
      601 tttaaagctc catgctatta atacatcacc aaattcccaa tggtcagatg atgaccctgt
      661 aatttttgtt gaaaagttaa tatacgatcg tgctttagat atatctagaa atgctgcaag
      721 aatggaaatg gaaagtggga attacaatac ttgtgaacta gcctatgcta cttcattatg
      781 gatgttagaa attttgctgg acgaaaattt tcaattcaac gaagtctatg atgacgagta
      841 tgcatcaaat atcacctcct tagacgaatc
//
