LOCUS       sequence-67770-e         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Falaleeva M, Sulsona CR, Zielke HR, Currey KM, de la Grange P, 
            Aslanzadeh V, Driscoll DJ, Stamm S
  TITLE     Molecular characterization of a patient presumed to have 
            prader-willi syndrome.
  JOURNAL   Clin Med Insights Case Rep. 2013 May 5;6:79-86. doi: 
            10.4137/CCRep.S11510. Print 2013.
  PUBMED    23700380
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Clin Med 
            Insights Case Rep."; date: "2013-05-5"; volume: "6:79-86. doi"; 
            pages: " 10.4137/CCRep.S11510. Print 2013"
COMMENT     Sequence Label: pCR2.1-SNORD116-3
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(336..354)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(337..354)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(372..388)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(372..388)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(385..407)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    396..412
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(420..426,427..444,445..450))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    465..486
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(603..620)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cactataggg cgaattgggc cctctagatg catgctcgag cggccgccag tgtgatggat
       61 atctgcagaa ttcgcccttt acccgtgaac cacaataagc caaagtggag gcaatgtgct
      121 ggacctcagt tccgatgaga acgacggtat gagttctcac tcattttgtt cagcttttcc
      181 aaggaatgtt tttatggggg actcatcatc gatccaagca cattcaaaga cgcaggatgt
      241 ttgacatcat aggctgaagg gcgaattcca gcacactggc ggccgttact agtggatccg
      301 agctcggtac caagcttgat gcatagcttg agtattctat agtgtcacct aaatagcttg
      361 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
      421 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      481 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      541 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      601 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      661 tcaaaggcgg taatacggtt atccacagaa t
//
