LOCUS       sequence-67772-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Ren X, Hurley JH
  TITLE     VHS domains of ESCRT-0 cooperate in high-avidity binding to 
            polyubiquitinated cargo.
  JOURNAL   EMBO J. 2010 Mar 17;29(6):1045-54. Epub 2010 Feb 11.
  PUBMED    20150893
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2010-03-17"; volume: "29(6)"; pages: "1045-54. Epub 2010 Feb 
            11"
COMMENT     Sequence Label: pGST1-Hrs-VHS
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..45
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(555..577)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(722..740)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        808..912
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catcctccaa aaatcgatac gaccgaaaac ctgtattttc agggcgccat ggggcgaggc
       61 agcggcacct tcgagcgtct cctagacaag gcgaccagcc agctcctgtt ggagacagat
      121 tgggagtcca ttttgcagat ctgcgacctg atccgccaag gggacacaca agcaaaatat
      181 gctgtgaatt ccatcaagaa gaaagtcaac gacaagaacc cacacgtcgc cttgtatgcc
      241 ctggaggtca tggaatctgt ggtaaagaac tgtggccaga cagttcatga tgaggtggcc
      301 aacaagcaga ccatggagga gctgaaggac ctgctgaaga gacaagtgga ggtaaacgtc
      361 cgtaacaaga tcctgtacct gatccaggcc tgggcgcatg ccttccggaa cgagcccaag
      421 tacaaggtgg tccaggacac ctaccagatc atgaaggtgg aggggcacgt ctttccataa
      481 gcctgcagtc tcgagcggcc gcatcgtgac tgactgacga tctgcctcgc gcgtttcggt
      541 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
      601 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
      661 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt ataattcttg aagacgaaag
      721 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
      781 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      841 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      901 aaaaggaaga gtatgagtat tcaacatttc c
//
