LOCUS       sequence-67773-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Ren X, Hurley JH
  TITLE     VHS domains of ESCRT-0 cooperate in high-avidity binding to 
            polyubiquitinated cargo.
  JOURNAL   EMBO J. 2010 Mar 17;29(6):1045-54. Epub 2010 Feb 11.
  PUBMED    20150893
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2010-03-17"; volume: "29(6)"; pages: "1045-54. Epub 2010 Feb 
            11"
COMMENT     Sequence Label: pGST2-yHse1-VHS
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..45
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(558..580)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(725..743)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        811..915
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catcctccaa aaatcgatac gaccgaaaac ctgtattttc agggcgccat gggatccatg
       61 tcatcatctg cgattaaaat aaggaatgca ctgttgaagg caacagatcc caagttgaga
      121 agtgataact ggcaatacat tttagatgtt tgcgatttgg tgaaggaaga tcccgaagat
      181 aatggacaag aggttatgtc tcttatagag aagaggctag aacaacagga tgctaacgtg
      241 atcttaagaa ccctctcttt gacggtatct ttggcagaaa attgtggttc ccgcttaaga
      301 caggaaatta gttcaaaaaa ttttacctct ttgttatatg cgctcatcga gagccattct
      361 gtacacatta ctctaaaaaa agcggtgact gatgttgtca agcaactgtc tgattcattc
      421 aaggatgacc catcattacg cgccatggga gatctatatg acaaaattaa aagaaaggct
      481 ccatatttgg tacagtaacg gccgcatcgt gactgactga cgatctgcct cgcgcgtttc
      541 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
      601 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
      661 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtataattc ttgaagacga
      721 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
      781 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      841 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      901 tgaaaaagga agagtatgag tat
//
