LOCUS       sequence-67775-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Ren X, Hurley JH
  TITLE     VHS domains of ESCRT-0 cooperate in high-avidity binding to 
            polyubiquitinated cargo.
  JOURNAL   EMBO J. 2010 Mar 17;29(6):1045-54. Epub 2010 Feb 11.
  PUBMED    20150893
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2010-03-17"; volume: "29(6)"; pages: "1045-54. Epub 2010 Feb 
            11"
COMMENT     Sequence Label: pGST1-yVps27-VHS
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..45
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(578..600)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(745..763)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catcctccaa aaatcgatac gaccgaaaac ctgtattttc agggcgccat gtccgttagc
       61 acgccaagtg agttagacgc attaatagaa caagccacta gtgagagcat tcccaacggt
      121 gaccttgatc tccccatagc gttggaaatt tcagatgtgt taagatctag aagggtaaat
      181 cccaaagatt ctatgcgttg tataaagaaa aggattctca atacggccga taatccaaat
      241 acccaattgt cttcctggaa gcttaccaat atatgtgtaa aaaatggtgg aacgcctttt
      301 attaaggaaa tttgttccag agagttcatg gataccatgg aacatgtcat tttaagagaa
      361 gatagcaacg aagaactttc tgagctggta aagaccatat tatatgaact atacgtagcc
      421 ttcaaaaatg attcgcaact taactatgtg gcaaaagttt atgataaatt aatctctcgt
      481 ggcattaaat tccctgaata agagcctgca gtctcgagcg gccgcatcgt gactgactga
      541 cgatctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
      601 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
      661 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
      721 tgtataattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
      781 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      841 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      901 gataaatgct tcaataatat tgaa
//
