LOCUS       sequence-67776-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Ren X, Hurley JH
  TITLE     VHS domains of ESCRT-0 cooperate in high-avidity binding to 
            polyubiquitinated cargo.
  JOURNAL   EMBO J. 2010 Mar 17;29(6):1045-54. Epub 2010 Feb 11.
  PUBMED    20150893
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2010-03-17"; volume: "29(6)"; pages: "1045-54. Epub 2010 Feb 
            11"
COMMENT     Sequence Label: pGST1-GGA1-VHS
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..45
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(572..594)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(739..757)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        825..929
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catcctccaa aaatcgatac gaccgaaaac ctgtattttc agggcgccat ggagcccgcg
       61 atggagccgg agactctgga ggcgcgaatc aatagagcca cgaaccccct gaacaaggag
      121 ctcgactggg ccagcatcaa cggcttctgc gagcagctca acgaggactt tgaggggcct
      181 ccactcgcca cccggctgct ggcccacaag atccagtccc cacaggagtg ggaggcgatc
      241 caggccttga cggtgctgga aacatgcatg aagagctgcg gcaagcggtt ccacgacgaa
      301 gtgggcaagt tccgctttct caacgagctc atcaaggtcg tgtctcccaa gtatctgggc
      361 tctcggacat cggagaaggt gaagaacaag atcttggagc tcctctacag ctggacagtg
      421 ggcctgcccg aggaggtgaa aatcgcagag gcctaccaga tgctaaagaa gcaggggatt
      481 gtaaagtccg actaagagcc tgcagtctcg agcggccgca tcgtgactga ctgacgatct
      541 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
      601 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
      661 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
      721 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
      781 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      841 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      901 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      961 ttcccttttt tgcggcattt tgccttcctg tttttg
//
