LOCUS       sequence-67780-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Ren X, Hurley JH
  TITLE     VHS domains of ESCRT-0 cooperate in high-avidity binding to 
            polyubiquitinated cargo.
  JOURNAL   EMBO J. 2010 Mar 17;29(6):1045-54. Epub 2010 Feb 11.
  PUBMED    20150893
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2010-03-17"; volume: "29(6)"; pages: "1045-54. Epub 2010 Feb 
            11"
COMMENT     Sequence Label: pGST1-GGA3-VHS
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..45
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(569..591)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(736..754)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        822..926
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catcctccaa aaatcgatac gaccgaaaac ctgtattttc agggcgccat ggcggaggcg
       61 gaaggggaaa gcctggagtc ctggctcaat aaagccacca atccttccaa ccgccaggag
      121 gactgggaat acataattgg cttctgtgat cagatcaaca aggagctgga agggccacag
      181 atcgccgtcc gactgctggc ccacaagatc cagtccccac aggaatggga ggcgctccag
      241 gccctgacgg tgctggaggc atgcatgaag aactgtggga ggagatttca taacgaagtg
      301 gggaagttcc gctttttgaa tgagttaatc aaagtcgtct ctccaaagta cctgggggac
      361 agggtgtctg agaaagtgaa gaccaaggtt attgagctgc tgtacagctg gaccatggcc
      421 ctgccagaag aagcaaagat caaagacgcc taccacatgc tgaagagaca gggcatagtg
      481 cagtctgact aagagcctgc agtctcgagc ggccgcatcg tgactgactg acgatctgcc
      541 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
      601 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      661 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtataatt
      721 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
      781 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      841 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      901 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      961 ccttttttgc ggcattttgc cttcctgttt ttgc
//
