LOCUS       sequence-67782-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Ren X, Hurley JH
  TITLE     VHS domains of ESCRT-0 cooperate in high-avidity binding to 
            polyubiquitinated cargo.
  JOURNAL   EMBO J. 2010 Mar 17;29(6):1045-54. Epub 2010 Feb 11.
  PUBMED    20150893
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2010-03-17"; volume: "29(6)"; pages: "1045-54. Epub 2010 Feb 
            11"
COMMENT     Sequence Label: pGST1-yGGA1-VHS
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..45
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(623..645)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(790..808)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catcctccaa aaatcgatac gaccgaaaac ctgtattttc agggcgccat gccacaaaga
       61 attgagctta cctccgaacc agtcagaaaa ccgcggtcta ctgagagctc actactcaga
      121 aagatacaaa gagcgtgtag atccacattg cctgaaccag atttgggctt gaacttagat
      181 gtagctgact atatcaactc aaagcaagga gcaaccccta gagaggctgt gttagcaatt
      241 gaaaaattgg tcaataatgg ggacacgcaa gcagctgttt ttgcactttc actactagac
      301 gtgctggtaa aaaattgtgg ttactctata catttgcaaa tctccaggaa ggaattctta
      361 aatgatctag tgaaaagatt tccagagcaa ccaccattga gatattccaa ggtgcaacaa
      421 atgattcttg aagccatcga ggaatggtac caaaccatct gcaaacatgc cagctacaaa
      481 gatgatcttc aatatatcaa cgacatgcat aaattactga aatacaaggg ttatactttc
      541 ccataagagc ctgcagtctc gagcggccgc atcgtgactg actgacgatc tgcctcgcgc
      601 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
      661 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
      721 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat aattcttgaa
      781 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
      841 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      901 tctaaataca ttcaaatatg tatccgctca tga
//
