LOCUS       sequence-67786-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Ren X, Hurley JH
  TITLE     VHS domains of ESCRT-0 cooperate in high-avidity binding to 
            polyubiquitinated cargo.
  JOURNAL   EMBO J. 2010 Mar 17;29(6):1045-54. Epub 2010 Feb 11.
  PUBMED    20150893
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2010-03-17"; volume: "29(6)"; pages: "1045-54. Epub 2010 Feb 
            11"
COMMENT     Sequence Label: pGST1-yGGA2-VHS
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..45
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(635..657)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(802..820)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catcctccaa aaatcgatac gaccgaaaac ctgtattttc agggcgccat gtcccatccg
       61 cactcacata gcatatattt gtctgagttg cccgtcagga aacctcaagc cttgggcaat
      121 cctcttttga ggaagattca aagagcttgc agaatgtcac ttgctgaacc agacttggcg
      181 ttgaatcttg acattgctga ctacattaat gaaaaacagg gcgctgcccc tagggatgct
      241 gccattgcac ttgctaaact gatcaacaat agagaatcgc acgtggcaat attcgcgtta
      301 tctctattag acgtgcttgt caagaattgt ggatacccat tccacttgca aatttctaga
      361 aaggaatttt tgaatgaact ggtcaagaga tttccaggtc acccaccatt acgttattct
      421 aagattcaga gattgatttt aactgctatt gaagaatggt atcaaacaat ttgtaaacac
      481 tcaagctaca agaatgatat gggttatatt agagacatgc accgtttgtt gaaatataag
      541 ggttatgcat tcccctaaga gcctgcagtc tcgagcggcc gcatcgtgac tgactgacga
      601 tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
      661 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
      721 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
      781 ataattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
      841 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      901 tttgtttatt tttctaaata cattcaaata tgtatccgct catg
//
