LOCUS       sequence-67787-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Ren X, Hurley JH
  TITLE     VHS domains of ESCRT-0 cooperate in high-avidity binding to 
            polyubiquitinated cargo.
  JOURNAL   EMBO J. 2010 Mar 17;29(6):1045-54. Epub 2010 Feb 11.
  PUBMED    20150893
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2010-03-17"; volume: "29(6)"; pages: "1045-54. Epub 2010 Feb 
            11"
COMMENT     Sequence Label: pGST1-TOM1-VHS
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..45
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(584..606)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(751..769)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        837..939
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catcctccaa aaatcgatac gaccgaaaac ctgtattttc agggcgccat ggactttctc
       61 ctggggaacc cgttcagctc tccagtggga cagcgcatcg agaaagccac agatggctcc
      121 ctgcagagcg aggactgggc cctcaacatg gagatctgcg acatcatcaa cgagacggag
      181 gaaggtccca aagatgccct ccgagcagta aagaagagaa tcgtggggaa taagaacttc
      241 cacgaggtga tgctggctct cacagtctta gaaacctgtg tcaagaactg cgggcaccgc
      301 ttccacgtgc tggtggccag ccaggacttc gtggagagtg tgctggtgag gaccatcctg
      361 cccaagaaca acccacccac catcgtgcat gacaaagtgc tcaacctcat ccagtcctgg
      421 gctgacgcgt tccgcagctc gcccgatctg acaggtgtgg tcaccatcta tgaggacctg
      481 cggaggaaag gcctggagtt cccctaagag cctgcagtct cgagcggccg catcgtgact
      541 gactgacgat ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
      601 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
      661 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
      721 gcggagtgta taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
      781 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
      841 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
      901 aaccctgata aatgcttcaa taatattgaa aaaggaaga
//
