LOCUS       sequence-67788-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Ren X, Hurley JH
  TITLE     VHS domains of ESCRT-0 cooperate in high-avidity binding to 
            polyubiquitinated cargo.
  JOURNAL   EMBO J. 2010 Mar 17;29(6):1045-54. Epub 2010 Feb 11.
  PUBMED    20150893
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2010-03-17"; volume: "29(6)"; pages: "1045-54. Epub 2010 Feb 
            11"
COMMENT     Sequence Label: pGST1-STAM1-UIM
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..45
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(260..282)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(427..445)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        513..617
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(836..855)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 catcctccaa aaatcgatac gaccgaaaac ctgtattttc agggcgccat ggctcttgta
       61 gccaaggatc ctggtactgt ggctaacaaa aaagaagaag aagatttagc aaaagccatt
      121 gagttgtctc tcaaggaaca aaggcagcag tcaaccaccc tttccacttt gtatccaagc
      181 taagagcctg cagtctcgag cggccgcatc gtgactgact gacgatctgc ctcgcgcgtt
      241 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
      301 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
      361 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtataat tcttgaagac
      421 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
      481 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
      541 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      601 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
      661 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
      721 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
      781 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
      841 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
      901 attctcagaa tgacttggtt gagtact
//
