LOCUS       sequence-67795-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Ren X, Hurley JH
  TITLE     VHS domains of ESCRT-0 cooperate in high-avidity binding to 
            polyubiquitinated cargo.
  JOURNAL   EMBO J. 2010 Mar 17;29(6):1045-54. Epub 2010 Feb 11.
  PUBMED    20150893
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2010-03-17"; volume: "29(6)"; pages: "1045-54. Epub 2010 Feb 
            11"
COMMENT     Sequence Label: pGST1-yVps27-tUIM
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..45
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(389..411)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(556..574)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        642..746
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catcctccaa aaatcgatac gaccgaaaac ctgtattttc agggcgccat gcataaaaga
       61 aagaaggaca gggactactc aactcctgag gatgaagaag agctgataag gaaagcaata
      121 gaactctcct tgaaagaatc tagaaatagc gctagtagcg agcctattgt ccccgttgta
      181 gaatcaaaga atgaggtgaa gagacaagaa atagaagaag aagaagatcc tgatttaaaa
      241 gctgctattc aggaaagctt gagagaagct gaagaagcga agctacgcag tgagcgtcaa
      301 aaagcctctt aagagcctgc agtctcgagc ggccgcatcg tgactgactg acgatctgcc
      361 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
      421 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      481 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtataatt
      541 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
      601 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      661 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      721 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      781 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      841 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      901 gtaagatcct tgagagtttt cgccccgaag aacgttttcc a
//
