LOCUS       sequence-67797-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Ren X, Hurley JH
  TITLE     Structural basis for endosomal recruitment of ESCRT-I by ESCRT-0 in 
            yeast.
  JOURNAL   EMBO J. 2011 Jun 1;30(11):2130-9. doi: 10.1038/emboj.2011.122. Epub 
            2011 Apr 19.
  PUBMED    21505419
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2011.122"; journalName: "EMBO J"; date: "2011-06-1- 
            1"; volume: "30"; issue: "11"; pages: "2130-9"
COMMENT     Sequence Label: pGST2-yVps23-UEV_C133A
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..45
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(608..630)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(775..793)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catcctccaa aaatcgatac gaccgaaaac ctgtattttc agggcgccat gtctgcaaac
       61 ggcaagatct ctgttcccga ggcggtggtc aattggctgt tcaaagtcat tcaacccatt
      121 tataacgacg gaagaacaac tttccacgat tctttagcgc tgctggacaa ttttcacagt
      181 ttaaggccaa gaacaagggt atttacgcac tctgacggca ctccacaact actgttgtcc
      241 atatatggta ccattagtac tggtgaggat ggttcatctc ctcacagtat tcctgtgatc
      301 atgtgggtac ccagtatgta cccggtaaaa cctcccttta taagtataaa cttggaaaat
      361 ttcgacatga acaccatcag tagttccttg cccattcaag aatacatcga ctccaatggg
      421 tggattgcgc tgcccatact ccacgcctgg gaccctgccg cgatgaacct tataatggtc
      481 gtacaagaat taatgagtct cttacacgag ccgccacaag atcaggcgta agagcctgca
      541 gtctcgagcg gccgcatcgt gactgactga cgatctgcct cgcgcgtttc ggtgatgacg
      601 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
      661 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
      721 ccatgaccca gtcacgtagc gatagcggag tgtataattc ttgaagacga aagggcctcg
      781 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      841 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      901 atatgtatcc gctcatga
//
