LOCUS       sequence-67814-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Fox JD, Routzahn KM, Bucher MH, Waugh DS
  TITLE     Maltodextrin-binding proteins from diverse bacteria and archaea are 
            potent solubility enhancers.
  JOURNAL   FEBS Lett. 2003 Feb 27. 537(1-3):53-7.
  PUBMED    12606030
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2003 Feb 27. 537(1-3):53-7."
COMMENT     Sequence Label: pKM1000
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(110..234)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(294..312)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      308..355
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(530..535,536..552,553..558))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(585..603)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        671..775
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
       61 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
      121 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
      181 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttcgatc
      241 ctaagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
      301 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
      361 aggaggaact atatccggat atcccgcaag aggcccggca gtaccggcat aaccaagcct
      421 atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc
      481 atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta
      541 tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct
      601 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
      661 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      721 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      781 tattcaacat ttccgtgtcg cccttattcc ct
//
