LOCUS       sequence-67815-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Fox JD, Routzahn KM, Bucher MH, Waugh DS
  TITLE     Maltodextrin-binding proteins from diverse bacteria and archaea are 
            potent solubility enhancers.
  JOURNAL   FEBS Lett. 2003 Feb 27. 537(1-3):53-7.
  PUBMED    12606030
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2003 Feb 27. 537(1-3):53-7."
COMMENT     Sequence Label: pKM1137
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacca tgaaaattga agaaggacaa ctcactattt ggatcaatgg
      181 tgataaaggc tataacggtt tggcggaagt cggtaagaag tttgaagccg acaccggaat
      241 caaagttact gtcgctcatc ctgatgcgct acaagataaa ttcccacaaa ccgcagcaac
      301 aggtgatggt cctgacattg tgttttgggc tcacgaccgt tttggcggtt atgcggaagc
      361 tggcctatta gtcgaaatca aaccttctgc aaaaattcaa gaaggcatcg tagatttcgc
      421 atgggatgca gtgaaataca acggcaaaat cattggttat ccaattgcgg tggaatcgtt
      481 atcactgatt tataacaaag accttgtccc taacccaccg aaaagctggg aagaagtcgc
      541 tgagctggat gccaaactga agaaagaagg caaatcggcc atcatgtgga acctgaaaga
      601 gccttacttc acttggcctt tgatggcggc tgatggcggt tacgcattca aatacggtgt
      661 tgatggctat gatgtgaaag acgcaggtat caacaataaa ggcgtaaaag acgcgatgaa
      721 cttcgtgaaa ggccttgtgg ataaaggcgt gatctctcca gatatggatt actcagtgtc
      781 tgagtctgcc tttaaccaag gtaataccgc gatgaccatc
//
