LOCUS       sequence-67817-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Fox JD, Routzahn KM, Bucher MH, Waugh DS
  TITLE     Maltodextrin-binding proteins from diverse bacteria and archaea are 
            potent solubility enhancers.
  JOURNAL   FEBS Lett. 2003 Feb 27. 537(1-3):53-7.
  PUBMED    12606030
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2003 Feb 27. 537(1-3):53-7."
COMMENT     Sequence Label: pKM1137
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(108..232)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(292..310)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      306..353
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(528..533,534..550,551..556))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(583..601)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        669..773
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
       61 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga
      121 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
      181 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatgggta
      241 aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
      301 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
      361 gaggaactat atccggatat cccgcaagag gcccggcagt accggcataa ccaagcctat
      421 gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat
      481 acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc
      541 gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat
      601 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
      661 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      721 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      781 ttcaacattt ccgtgtcgcc cttattc
//
