LOCUS       sequence-67818-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Cortical dynein and asymmetric membrane elongation coordinately 
            position the spindle in anaphase.
  JOURNAL   Cell. 2013 Jul 18;154(2):391-402. doi: 10.1016/j.cell.2013.06.010.
  PUBMED    23870127
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.06"; journalName: "Cell"; date: "2013-07-18- 
            18"; volume: "154"; issue: "2"; pages: "391-402"
COMMENT     Sequence Label: pTK50_GFP-NuMA-N
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..71
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             84..128
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gtcagtccgg aggggatcgg tggagctcca ccggtggcgg ccgctctaga gagaatcttt
       61 attttcaggg cgccgccaaa ttcaaagaaa ccgctgctgc taaattcgaa cgccagcaca
      121 tggacagcgg aggtggaggt actagtctcg acatgacact ccacgccacc cggggggctg
      181 cactcctctc ttgggtgaac agtctacacg tggctgaccc tgtggaggct gtgctgcagc
      241 tccaggactg cagcatcttc atcaagatca ttgacagaat ccatggcact gaagagggac
      301 agcaaatctt gaagcagccg gtgtcagaga gactggactt tgtgtgcagt tttctgcaga
      361 aaaatcgaaa acatccctct tccccagaat gcctggtatc tgcacagaag gtgctagagg
      421 gatcagagct ggaactggcg aagatgacca tgctgctctt ataccactct accatgagct
      481 ccaaaagtcc cagggactgg gaacagtttg aatataaaat tcaggctgag ttggctgtca
      541 ttcttaaatt tgtgctggac catgaggacg ggctaaacct taatgaggac ctagagaact
      601 tcctacagaa agctcctgtg ccttctacct gttctagcac attccctgaa gagctctccc
      661 cacctagcca ccaggccaag agggagattc gcttcctaga gctacagaag gttgcctcct
      721 cttccagtgg gaacaacttt ctctcaggtt ctccagcttc tcccatgggt gatatcctgc
      781 agaccccaca gtaagaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatct
      841 agataactga tcataatcag ccatacca
//
