LOCUS       sequence-67819-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Kishore S, Khanna A, Stamm S
  TITLE     Rapid generation of splicing reporters with pSpliceExpress.
  JOURNAL   Gene. 2008 Dec 31;427(1-2):104-10. Epub 2008 Oct 1.
  PUBMED    18930792
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2008-12-31"; volume: "427(1-2)"; pages: "104-10. Epub 2008 
            Oct 1"
COMMENT     Sequence Label: pDESTsplice
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(86..92,93..110,111..116))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(141..265)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(292..308)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(293..309)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
       61 gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag tattatgtag
      181 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
      241 gttcagcttt tttgtacaaa cttgtttgat gggctgcagg aattagctta tcgataccgt
      301 cgacctcgag ggggggcccg gtacctatcc tggggcctag ccacccgtca ccacctagga
      361 gacttaagga aggattctca ctccttcctg gcagctacca tgtttacata tgtctagctg
      421 cccacagaaa tgtgcccccc actggttagc ctgagaccct ccacacctag ggcacaattt
      481 acaaggtctc aagggtgaaa gacaaacagg gtagttagca cttgggatag aattaattca
      541 ggagcagagc ttaccttgtg ggtcctccac ttcgcggcgg gacatgggtg tgtagaagaa
      601 tccacgctcc ccacacacca ggtagagagc ttccaccaag tgagaaccac aaaggtgctg
      661 tttgacaaaa gcctgggcag ggcggggctc ccagaggatg agcagggcca gcaggggcag
      721 gaagcggatc gatcctggca gctgtcaccg tagacacccc catcccatcg cccaggaaga
      781 tgatgaggtt cttggcggct gtctgtgcag gctgcagctt
//
