LOCUS       sequence-67822-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Kishore S, Khanna A, Stamm S
  TITLE     Rapid generation of splicing reporters with pSpliceExpress.
  JOURNAL   Gene. 2008 Dec 31;427(1-2):104-10. Epub 2008 Oct 1.
  PUBMED    18930792
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2008-12-31"; volume: "427(1-2)"; pages: "104-10. Epub 2008 
            Oct 1"
COMMENT     Sequence Label: pDESTsplice
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(108..232)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(242..258)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(242..258)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
       61 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga
      121 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
      181 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gttcgatggg
      241 ggatccacta gttctagagc ggccgccacc gcggtggagc tcggtaccta tttggggacc
      301 ccatagagca ctgcactgac tgagggatgg taacaggatg tgtaggtttt ggaggcccat
      361 atgtccattc atgaccagtg acttgtctca cagccatgca acccttgcct cctgtgctga
      421 cttagcaggg gataaagtga gagaaagcct gggctaatca gggggtcgct cagctcctcc
      481 taactggatt gtcctatgtg tctttgcttc tgtgctgctg atgctctgcc ctgtgctgac
      541 atgacctccc tggcagtggc acaactggag ctgggtggag gcccgtgacc ttcagacctt
      601 ggcactggag gtggcccggc agaagcgcgg catcgtggat cagtgctgca ccagcatctg
      661 ctctctctac caactggaga actactgcaa ctaggcccac cactaccctg tccacccctc
      721 tgcaatgaat aaaacctttg aaagagcact acaagttgtg tgtacatgcg tgcatgtgca
      781 tatgtggtgc ggggg
//
