LOCUS       sequence-67824-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Cortical dynein and asymmetric membrane elongation coordinately 
            position the spindle in anaphase.
  JOURNAL   Cell. 2013 Jul 18;154(2):391-402. doi: 10.1016/j.cell.2013.06.010.
  PUBMED    23870127
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.06"; journalName: "Cell"; date: "2013-07-18- 
            18"; volume: "154"; issue: "2"; pages: "391-402"
COMMENT     Sequence Label: pTK51_GFP-NuMA-C
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             79..123
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 tccggagggg atcggtggag ctccaccggt ggcggccgct ctagagagaa tctttatttt
       61 cagggcgccg ccaaattcaa agaaaccgct gctgctaaat tcgaacgcca gcacatggac
      121 agcggaggtg gaggtactag tctcgacttc caggtggcaa ctgatgcttt aaagagccgt
      181 gagccccagg ctaagcccca gctggacttg agtattgaca gcctggatct gagctgcgag
      241 gaggggaccc cactcagtat caccagcaag ctgcctcgta cccagccaga cggcaccagc
      301 gtccctggag aaccagcctc acctatctcc cagcgcctgc cccccaaggt agaatccctg
      361 gagagtctct acttcactcc catccctgct cggagtcagg cccccctgga gagcagcctg
      421 gactccctgg gagacgtctt cctggactcg ggtcgtaaga cccgctccgc tcgtcggcgc
      481 accacgcaga tcatcaacat caccatgacc aagaagctag atgtggaaga gccagacagc
      541 gccaactcat cgttctacag cacgcggtct gctcctgctt cccaggctag cctgcgagcc
      601 acctcctcta ctcagtctct agctcgcctg ggttctcccg attatggcaa ctcagccctg
      661 ctcagcttgc ctggctaccg ccccaccact cgcagttctg ctcgtcgttc ccaggccggg
      721 gtgtccagtg gggcccctcc aggaaggaac agcttctaca tgggcacttg ccaggatgag
      781 cctgagcagc tggatgactg gaaccgcatt gcagagctgc agcagcgcaa tcgagtgtgc
      841 cccccacatc tgaagacctg ctatcccctg ga
//
