LOCUS       sequence-67826-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Pennacchio LA, Ahituv N, Moses AM, Prabhakar S, Nobrega MA, Shoukry 
            M, Minovitsky S, Dubchak I, Holt A, Lewis KD, Plajzer-Frick I, 
            Akiyama J, De Val S, Afzal V, Black BL, Couronne O, Eisen MB, Visel 
            A, Rubin EM
  TITLE     In vivo enhancer analysis of human conserved non-coding sequences.
  JOURNAL   Nature. 2006 Nov 23;444(7118):499-502. Epub 2006 Nov 5.
  PUBMED    17086198
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2006-11-23"; volume: "444(7118)"; pages: "499-502. Epub 2006 
            Nov 5"
COMMENT     Sequence Label: Hsp68-LacZ-Gateway
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(81..87,88..105,106..111))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(136..260)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(285..301)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(286..302)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(330..348)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(369..385)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(369..385)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(382..404)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    393..409
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(417..423,424..441,442..447))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    462..483
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(600..617)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgacgg
       61 atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa agcctggggt
      121 gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
      181 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
      241 gcttttttgt acaaacttgt gatggaattc gatatcaagc ttatcgatac cgtcgacctc
      301 gagggggggc ccggtaccag cttttgttcc ctttagtgag ggttaatttc gagcttggcg
      361 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      421 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      481 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      541 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
      601 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      661 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      721 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttcc
//
