LOCUS       sequence-67831-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Pennacchio LA, Ahituv N, Moses AM, Prabhakar S, Nobrega MA, Shoukry 
            M, Minovitsky S, Dubchak I, Holt A, Lewis KD, Plajzer-Frick I, 
            Akiyama J, De Val S, Afzal V, Black BL, Couronne O, Eisen MB, Visel 
            A, Rubin EM
  TITLE     In vivo enhancer analysis of human conserved non-coding sequences.
  JOURNAL   Nature. 2006 Nov 23;444(7118):499-502. Epub 2006 Nov 5.
  PUBMED    17086198
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2006-11-23"; volume: "444(7118)"; pages: "499-502. Epub 2006 
            Nov 5"
COMMENT     Sequence Label: Hsp68-LacZ-Gateway
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..40
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     25..41
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    66..190
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(215..220,221..238,239..245)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(365..384)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aaaagctggt accgggcccc ccctcgaggt cgacggtatc gataagcttg atatcgaatt
       61 ccatcacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata aatatcaata
      121 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc
      181 agtcactatg gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      241 taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat
      301 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca
      361 ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat
      421 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca
      481 cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga
      541 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
      601 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
      661 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
      721 tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
      781 caatatggac aacttcttcg cccc
//
