LOCUS       sequence-67832-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Cortical dynein and asymmetric membrane elongation coordinately 
            position the spindle in anaphase.
  JOURNAL   Cell. 2013 Jul 18;154(2):391-402. doi: 10.1016/j.cell.2013.06.010.
  PUBMED    23870127
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.06"; journalName: "Cell"; date: "2013-07-18- 
            18"; volume: "154"; issue: "2"; pages: "391-402"
COMMENT     Sequence Label: pTK52_GFP-NuMA-C-3A
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..68
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             81..125
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 agtccggagg ggatcggtgg agctccaccg gtggcggccg ctctagagag aatctttatt
       61 ttcagggcgc cgccaaattc aaagaaaccg ctgctgctaa attcgaacgc cagcacatgg
      121 acagcggagg tggaggtact agtctcgact tccaggtggc aactgatgct ttaaagagcc
      181 gtgagcccca ggctaagccc cagctggact tgagtattga cagcctggat ctgagctgcg
      241 aggaggggac cccactcagt atcaccagca agctgcctcg tacccagcca gacggcacca
      301 gcgtccctgg agaaccagcc tcacctatct cccagcgcct gccccccaag gtagaatccc
      361 tggagagtct ctacttcact cccatccctg ctcggagtca ggcccccctg gagagcagcc
      421 tggactccct gggagacgtc ttcctggact cgggtcgtaa gacccgctcc gctcgtcggc
      481 gcaccacgca gatcatcaac atcaccatga ccaagaagct agatgtggaa gagccagaca
      541 gcgccaactc atcgttctac agcacgcggt ctgctcctgc ttcccaggct agcctgcgag
      601 ccacctcctc tactcagtct ctagctcgcc tgggttctcc cgattatggc aactcagccc
      661 tgctcagctt gcctggctac cgccccacca ctcgcagttc tgctcgtcgt tcccaggccg
      721 gggtgtccag tggggcccct ccaggaagga acagcttcta catgggcact tgccaggatg
      781 agcctgagca gctggatgac tggaaccgca ttgcagagct gcagcagcgc aatcgagtgt
      841 gccccccaca tctg
//
