LOCUS       sequence-67847-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Cortical dynein and asymmetric membrane elongation coordinately 
            position the spindle in anaphase.
  JOURNAL   Cell. 2013 Jul 18;154(2):391-402. doi: 10.1016/j.cell.2013.06.010.
  PUBMED    23870127
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.06"; journalName: "Cell"; date: "2013-07-18- 
            18"; volume: "154"; issue: "2"; pages: "391-402"
COMMENT     Sequence Label: pTK64_GFP-NuMA-Bonsai
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..73
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             86..130
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 ctgtcagtcc ggaggggatc ggtggagctc caccggtggc ggccgctcta gagagaatct
       61 ttattttcag ggcgccgcca aattcaaaga aaccgctgct gctaaattcg aacgccagca
      121 catggacagc ggaggtggag gtactagtct cgacatgaca ctccacgcca cccggggggc
      181 tgcactcctc tcttgggtga acagtctaca cgtggctgac cctgtggagg ctgtgctgca
      241 gctccaggac tgcagcatct tcatcaagat cattgacaga atccatggca ctgaagaggg
      301 acagcaaatc ttgaagcagc cggtgtcaga gagactggac tttgtgtgca gttttctgca
      361 gaaaaatcga aaacatccct cttccccaga atgcctggta tctgcacaga aggtgctaga
      421 gggatcagag ctggaactgg cgaagatgac catgctgctc ttataccact ctaccatgag
      481 ctccaaaagt cccagggact gggaacagtt tgaatataaa attcaggctg agttggctgt
      541 cattcttaaa tttgtgctgg accatgagga cgggctaaac cttaatgagg acctagagaa
      601 cttcctacag aaagctcctg tgccttctac ctgttctagc acattccctg aagagctctc
      661 cccacctagc caccaggcca agagggagat tcgcttccta gagctacaga aggttgcctc
      721 ctcttccagt gggaacaact ttctctcagg ttctccagct tctcccatgg gtgatatcct
      781 gcagacccca cagttccaga
//
