LOCUS       sequence-67854-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of PKI (PKA inhibitor).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  TITLE     RSV-PKI
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRSV-PKI-v2
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(274..290)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(274..290)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     intron          376..441
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             571..591
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cattggtgtg cacctccaag cttgccacca tgggaactga tgtcgaaact acttatgccg
       61 attttatcgc ctcaggacgt actggtcgac gtaacgccat ccacgatatc ctggtctcga
      121 gtgcttccgg aaactcaaac gaactggccc tgaagctggc tggtctagat attaacaaaa
      181 cagaaggaga ggaagatgct caaagatctt caactgaaca atccggagaa gctcaaggag
      241 aagctgctaa gtccgagtca tagtagggat ctggatccac tagttctaga gcggccgcag
      301 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
      361 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
      421 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
      481 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
      541 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
      601 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
      661 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
      721 atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca taacatactg
      781 ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg
      841 tgtaccttta gctttttaat ttgtaaaggg gttaataag
//
