LOCUS       sequence-67855-e        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of intactive PKI (PKA inhibitor) mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  TITLE     RSV-PKI
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRSV-PKImut-v2
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(267..283)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(267..283)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     intron          369..434
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             564..584
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cattggtgtg cacctccagc ttgccaccat gggaactgat gtcgaaacta cttatgccga
       61 ttttatcgcc tcaggacgta ctggtggagg taacgccatc cacgatatcc tggtctcgag
      121 tgcttccgga aactcaaacg aactggccct gaagctggct ggtctagata ttaacaaaac
      181 agaaggagag gaagatgctc aaagatcttc aactgaacaa tccggagaag ctcaaggaga
      241 agctgctaag tccgagtcat agtagggatc cactagttct agagcggccg cagatctttg
      301 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
      361 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
      421 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct
      481 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg
      541 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact
      601 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
      661 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa
      721 aatattctgt aacctttata agtaggcata acagttataa tcataacata ctgttttttc
      781 ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct
      841 ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta
      901 gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
      961 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     1021 gcagcttata atggttacaa
//
