LOCUS       sequence-67856-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter gene for factors with GAL4 DNA binding domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Sun P, Enslen H, Myung PS, Maurer RA
  TITLE     Differential activation of CREB by Ca2+/calmodulin-dependent protein
            kinases type II and type IV involves phosphorylation of a site that 
            negatively regulates activity.
  JOURNAL   Genes Dev. 1994 Nov 1;8(21):2527-39.
  PUBMED    7958915
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "1994-11-1"; volume: "8(21)"; pages: "2527-39"
COMMENT     Sequence Label: 5xGAL4-TATA-luciferase
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    131..225
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     polyA_signal    262..396
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    490..624
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(755..777)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 agaatggcgc cgggcctttc tttatgtttt tggcgtcttc catggtggca acagtaccgg
       61 aatgccaagc ttgcatgcct gcaggtcgac tggctagcca ggatccatta tataccctct
      121 agagtctccg ctcggaggac agtactccgc tcggaggaca gtactccgct cggaggacag
      181 tactccgctc ggaggacagt actccgctcg gaggacagta ctccgacctg caggcatgca
      241 agctggtacc gagctcgaat tgatccagac atgataagat acattgatga gtttggacaa
      301 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
      361 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaattgcat tcattttatg
      421 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
      481 gatatggctg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
      541 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
      601 attataagct gcaataaaca agttaacaac aattgcattc attttatgtt tcaggttcag
      661 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtga tatggctgat
      721 cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
      781 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcac
      841 cgggtgttgt cgggtgtcgt ggcgcagcca
//
