LOCUS       sequence-67861-e        8105 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  control FRET sensor for beta2-adrenergic receptor .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8105)
  AUTHORS   Malik RU, Ritt M, Devree BT, Neubig RR, Sunahara RK, 
            Sivaramakrishnan S
  TITLE     Detection of G Protein-selective G Protein-coupled Receptor (GPCR) 
            Conformations in Live Cells.
  JOURNAL   J Biol Chem. 2013 Jun 14;288(24):17167-78. doi: 
            10.1074/jbc.M113.464065. Epub 2013 Apr 29.
  PUBMED    23629648
REFERENCE   2  (bases 1 to 8105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.464065"; journalName: "J Biol Chem"; date: 
            "2013-06-14- 14"; volume: "288"; issue: "24"; pages: "17167-78"
COMMENT     Sequence Label: pCDNA_B2AR-no-pep
FEATURES             Location/Qualifiers
     source          1..8105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             920..946
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(2237..2239,2240..2950)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2237..2239,2240..2950)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             2975..2995
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(3251..3253,3254..3964)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4057..4074)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4063..4287
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4420..4439)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4571..4618
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4626..5648
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4626..5648
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5778..5899
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5815..5834)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5869..5888
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5948..5964)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5948..5964)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5961..5983)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5972..5988
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5996..6002,6003..6020,6021..6026))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6041..6062
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6179..6196)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6350..6938)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6430..6449)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7109..7900,7901..7969))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7732..7751
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7970..8074)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttatgt atccttatga tgttcctgat tacgctggaa gtggaatggg
      961 gcaacccggg aacggcagcg ccttcttgct ggcacccaat agaagccatg cgccggacca
     1021 cgacgtcacg cagcaaaggg acgaggtgtg ggtggtgggc atgggcatcg tcatgtctct
     1081 catcgtcctg gccatcgtgt ttggcaatgt gctggtcatc acagccattg ccaagttcga
     1141 gcgtctgcag acggtcacca actacttcat cacttcactg gcctgtgctg atctggtcat
     1201 gggcctggca gtggtgccct ttggggccgc ccatattctt atgaaaatgt ggacttttgg
     1261 caacttctgg tgcgagtttt ggacttccat tgatgtgctg tgcgtcacgg ccagcattga
     1321 gaccctgtgc gtgatcgcag tggatcgcta ctttgccatt acttcacctt tcaagtacca
     1381 gagcctgctg accaagaata aggcccgggt gatcattctg atggtgtgga ttgtgtcagg
     1441 ccttacctcc ttcttgccca ttcagatgca ctggtaccgg gccacccacc aggaagccat
     1501 caactgctat gccaatgaga cctgctgtga cttcttcacg aaccaagcct atgccattgc
     1561 ctcttccatc gtgtccttct acgttcccct ggtgatcatg gtcttcgtct actccagggt
     1621 ctttcaggag gccaaaaggc agctccagaa gattgacaaa tctgagggcc gcttccatgt
     1681 ccagaacctt agccaggtgg agcaggatgg gcggacgggg catggactcc gcagatcttc
     1741 caagttctgc ttgaaggagc acaaagccct caagacgtta ggcatcatca tgggcacttt
     1801 caccctctgc tggctgccct tcttcatcgt taacattgtg catgtgatcc aggataacct
     1861 catccgtaag gaagtttaca tcctcctaaa ttggataggc tatgtcaatt ctggtttcaa
     1921 tccccttatc tactgccgga gcccagattt caggattgcc ttccaggagc ttctgtgcct
     1981 gcgcaggtct tctttgaagg cctatgggaa tggctactcc agcaacggca acacagggga
     2041 gcagagtgga tatcacgtgg aacaggagaa agaaaataaa ctgctgtgtg aagacctccc
     2101 aggcacggaa gactttgtgg gccatcaagg tactgtgcct agcgataaca ttgattcaca
     2161 agggaggaat tgtagtacaa atgactcact gctgtctaga ggaagcggag gaagcggagg
     2221 aagcggagga agcggagtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     2281 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     2341 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     2401 gccctggccc accctcgtga ccaccttcgg ctacggcctg atgtgcttcg cccgctaccc
     2461 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     2521 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     2581 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     2641 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     2701 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     2761 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     2821 gcccgacaac cactacctga gctaccagtc caaactgagc aaagacccca acgagaagcg
     2881 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     2941 gctgtacaag gaattcggaa gcggaggaag cggagaaaac ctgtattttc agggcggaag
     3001 cggagaagag gaagagaaaa aaaaacagca ggaagaggaa gcagaaaggc tgaggcgtat
     3061 tcaagaagaa atggaaaagg aaagaaaaag acgtgaagaa gatgaacaac gtcgaagaaa
     3121 ggaggaggag gaaaggcgga tgaaacttga gatggaagca aagagaaaac aagaagaaga
     3181 agagagaaag aaaagggaag atgatgaaaa acgcaagaag aagggctctg gcggctctgg
     3241 cggcgcgccc gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     3301 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     3361 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     3421 gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcgcccgct accccgacca
     3481 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     3541 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     3601 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3661 ggggcacaag ctggagtaca acgccatcag cgacaacgtc tatatcaccg ccgacaagca
     3721 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3781 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3841 caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca
     3901 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3961 caagttaatt aacggaagcg gaggaagcgg aggaagcgga ggaagcggat gagcggccgc
     4021 tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     4081 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     4141 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     4201 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     4261 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     4321 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4381 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4441 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4501 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4561 cacgtaccta gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcct
     4621 tggccaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg
     4681 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg
     4741 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag
     4801 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca
     4861 ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt
     4921 tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg
     4981 atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag
     5041 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt
     5101 atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg
     5161 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg
     5221 gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg
     5281 cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg
     5341 cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc
     5401 cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg
     5461 acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg
     5521 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg
     5581 gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa
     5641 aggaatagca cgtactacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     5701 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     5761 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5821 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5881 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     5941 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6001 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6061 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6121 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6181 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6241 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6301 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6361 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6421 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6481 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6541 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6601 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6661 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6721 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6781 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6841 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6901 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6961 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7021 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7081 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7141 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7201 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7261 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7321 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7381 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7441 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7501 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7561 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7621 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7681 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7741 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7801 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7861 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7921 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7981 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8041 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8101 acgtc
//
