LOCUS       sequence-67872-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Cortical dynein and asymmetric membrane elongation coordinately 
            position the spindle in anaphase.
  JOURNAL   Cell. 2013 Jul 18;154(2):391-402. doi: 10.1016/j.cell.2013.06.010.
  PUBMED    23870127
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.06"; journalName: "Cell"; date: "2013-07-18- 
            18"; volume: "154"; issue: "2"; pages: "391-402"
COMMENT     Sequence Label: pTK82_GFP-4.1G
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             60..80
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             93..137
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 acgagctgta caagtccgga ggggatcggt ggagctccac cggtggcggc cgctctagag
       61 agaatcttta ttttcagggc gccgccaaat tcaaagaaac cgctgctgct aaattcgaac
      121 gccagcacat ggacagcgga ggtggaggta ctagtctcga gatgactact gaagtaggct
      181 ctgtgtctga agtgaagaag gactctagcc agttaggaac agatgcaacc aaggaaaaac
      241 ctaaagaagt agcagaaaat cagcagaatc agtcttccga tccagaggag gaaaaaggtt
      301 cccagccacc tcctgcagct gaaagccaaa gtagtctacg ccgccagaag agagagaagg
      361 aaacatcgga gagcaggggt atttctcggt tcataccgcc atggcttaag aagcaaaagt
      421 catatacctt agtagtggcc aaagatggag gagataaaaa agagcctacc caagctgttg
      481 ttgaagaaca ggtcttagat aaagaggaac cccttccaga agaacagaga caggctaagg
      541 gtgatgctga agaaatggct cagaagaaac aagagattaa agttgaagtc aaggaagaaa
      601 aaccctcagt gagcaaggaa gaaaaaccct cagtgagcaa agtggagatg cagcctactg
      661 aattagtaag taaggagaga gaagagaagg taaaagaaac acaggaagac aaattagaag
      721 gaggagcagc aaaaagggag accaaggaag tgcagaccaa tgagctgaaa gcagagaagg
      781 catctcaaaa agtcaccaag aagaccaaaa ctgtccagtg taaagtgacc ctcttagatg
      841 gcaccgaata cagctgtgac ctgga
//
