LOCUS       sequence-67874-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Cortical dynein and asymmetric membrane elongation coordinately 
            position the spindle in anaphase.
  JOURNAL   Cell. 2013 Jul 18;154(2):391-402. doi: 10.1016/j.cell.2013.06.010.
  PUBMED    23870127
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.06"; journalName: "Cell"; date: "2013-07-18- 
            18"; volume: "154"; issue: "2"; pages: "391-402"
COMMENT     Sequence Label: pTK88_GFP-Anillin
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..78
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             91..135
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gagctgtaca agtccggagg ggatcggtgg agctccaccg gtggcggccg ctctagagag
       61 aatctttatt ttcagggcgc cgccaaattc aaagaaaccg ctgctgctaa attcgaacgc
      121 cagcacatgg acagcggagg tggaggtact agtctcgaga tggatccgtt tacggagaaa
      181 ctgctggagc gaacccgtgc caggcgagag aatcttcaga gaaaaatggc tgagaggccc
      241 acagcagctc caaggtctat gactcatgct aagcgagcta gacagccact ttcagaagca
      301 agtaaccagc agcccctctc tggtggtgaa gagaaatctt gtacaaaacc atcgccatca
      361 aaaaaacgct gttctgacaa cactgaagta gaagtttcta acttggaaaa taaacaacca
      421 gttgagtcga catctgcaaa atcttgttct ccaagtcctg tgtctcctca ggtgcagcca
      481 caagcagcag ataccatcag tgattctgtt gctgtcccgg catcactgct gggcatgagg
      541 agagggctga actcaagatt ggaagcaact gcagcctcct cagttaaaac acgtatgcaa
      601 aaacttgcag agcaacggcg ccgttgggat aatgatgata tgacagatga cattcctgaa
      661 agctcactct tctcaccaat gccatcagag gaaaaggctg cttcccctcc cagacctctg
      721 ctttcaaatg cctcggcaac tccagttggc agaaggggcc gtctggccaa tcttgctgca
      781 actatttgct cctgggaaga tgatgtaaat cactcatttg caaaacaaaa cagtgtacaa
      841 gaacagcctg gtaccgcttg tttatc
//
