LOCUS       sequence-67882-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Cortical dynein and asymmetric membrane elongation coordinately 
            position the spindle in anaphase.
  JOURNAL   Cell. 2013 Jul 18;154(2):391-402. doi: 10.1016/j.cell.2013.06.010.
  PUBMED    23870127
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.06"; journalName: "Cell"; date: "2013-07-18- 
            18"; volume: "154"; issue: "2"; pages: "391-402"
COMMENT     Sequence Label: pTK96_mCherry-MRLC2
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             294..314
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             327..371
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
       61 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
      121 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
      181 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
      241 tgtacaagtc cggaggggat cggtggagct ccaccggtgg cggccgctct agagagaatc
      301 tttattttca gggcgccgcc aaattcaaag aaaccgctgc tgctaaattc gaacgccagc
      361 acatggacag cggaggtgga ggtactagtc tcgagatgtc gagcaaaaag gcaaagacca
      421 agaccaccaa gaagcgccct cagcgtgcaa catccaatgt gtttgccatg tttgaccagt
      481 cacagattca ggagttcaaa gaggccttca acatgattga tcagaacaga gatggcttca
      541 tcgacaagga agatttgcat gatatgcttg cttctctagg gaagaatccc actgatgcat
      601 accttgatgc catgatgaat gaggccccag ggcccatcaa tttcaccatg ttcctgacca
      661 tgtttggtga gaagttaaat ggcacagatc ctgaagatgt catcagaaac gcctttgctt
      721 gctttgatga agaagcaaca ggcaccattc aggaagatta cctaagagag ctgctgacaa
      781 ccatggggga tcggtttaca gatgaggaag tggatgagct gtacagagaa gcacctattg
      841 acaaaaaggg gaatttcaat tacatcgagt tcacacgcat cct
//
