LOCUS       sequence-67883-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  visualizes chromosomes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Cortical dynein and asymmetric membrane elongation coordinately 
            position the spindle in anaphase.
  JOURNAL   Cell. 2013 Jul 18;154(2):391-402. doi: 10.1016/j.cell.2013.06.010.
  PUBMED    23870127
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.06"; journalName: "Cell"; date: "2013-07-18- 
            18"; volume: "154"; issue: "2"; pages: "391-402"
COMMENT     Sequence Label: pTK168_mCherry-H2B
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             289..309
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             322..366
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             391..771
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSDK"
ORIGIN
        1 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
       61 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
      121 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
      181 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
      241 aagtccggag gggatcggtg gagctccacc ggtggcggcc gctctagaga gaatctttat
      301 tttcagggcg ccgccaaatt caaagaaacc gctgctgcta aattcgaacg ccagcacatg
      361 gacagcggag gtggaggtac tagtctcgag atgccagagc cagcgaagtc tgctcccgcc
      421 ccgaaaaagg gctccaagaa ggcggtgact aaggcgcaga agaaaggcgg caagaagcgc
      481 aagcgcagcc gcaaggagag ctattccatc tatgtgtaca aggttctgaa gcaggtccac
      541 cctgacaccg gcatttcgtc caaggccatg ggcatcatga attcgtttgt gaacgacatt
      601 ttcgagcgca tcgcaggtga ggcttcccgc ctggcgcatt acaacaagcg ctcgaccatc
      661 acctccaggg agatccagac ggccgtgcgc ctgctgctgc ctggggagtt ggccaagcac
      721 gccgtgtccg agggtactaa ggccatcacc aagtacacca gcgataagta gccgcggtgg
      781 cggtctggac cacgccggag agcgtcgaag cggggggcgg tgttcgccga gatcggcccg
      841 cgcatggc
//
