LOCUS       sequence-67884-e         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of query pri-miRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 V5-DEST mir-1-1 reporter
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(37..139)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(317..441)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
       61 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
      121 acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt
      181 atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta
      241 ttcggcgcaa agtgcgtcgg gtgatgctgc caacttagtc gactacaggt cactaatacc
      301 atctaagtag ttgattcata gtgactggat atgttgtgtt ttacagtatt atgtagtctg
      361 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
      421 agctttcttg tacaaagtgg ttgatctaga gggcccgcag tggggtcagc ttctaccggg
      481 gcggcgtccc ggggtcttgg aactgcatgc agactgcctg cttgggaaac atacttcttt
      541 atatgcccat atggacctgc taagctatgg aatgtaaaga agtatgtatc tcaggccggg
      601 acctctctcg ccgcactgag gggcactcca caccacgggg gccgccgcgg ccgacagcca
      661 gccaggcggc tgtggtgggg gtga
//
