LOCUS       sequence-67885-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Cortical dynein and asymmetric membrane elongation coordinately 
            position the spindle in anaphase.
  JOURNAL   Cell. 2013 Jul 18;154(2):391-402. doi: 10.1016/j.cell.2013.06.010.
  PUBMED    23870127
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.06"; journalName: "Cell"; date: "2013-07-18- 
            18"; volume: "154"; issue: "2"; pages: "391-402"
COMMENT     Sequence Label: pCB150-2_GFP-EB1
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..78
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             91..135
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gagctgtaca agtccggagg ggatcggtgg agctccaccg gtggcggccg ctctagagag
       61 aatctttatt ttcagggcgc cgccaaattc aaagaaaccg ctgctgctaa attcgaacgc
      121 cagcacatgg acagcggagg tggaggtact agtctcgaga tggcagtgaa cgtatactca
      181 acgtcagtga ccagtgataa cctaagtcga catgacatgc tggcctggat caatgagtct
      241 ctgcagttga atctgacaaa gatcgaacag ttgtgctcag gggctgcgta ttgtcagttt
      301 atggacatgc tgttccctgg ctccattgcc ttgaagaaag tgaaattcca agctaagcta
      361 gaacacgagt acatccagaa cttcaaaata ctacaagcag gttttaagag aatgggtgtt
      421 gacaaaataa ttcctgtgga caaattagta aaaggaaagt ttcaggacaa ttttgaattc
      481 gttcagtggt tcaagaagtt tttcgatgca aactatgatg gaaaagacta tgaccctgtg
      541 gctgccagac aaggtcaaga aactgcagtg gctccttccc ttgttgctcc agctctgaat
      601 aaaccgaaga aacctctcac ttctagcagt gcagctcccc agaggcccat ctcaacacag
      661 agaaccgctg cggctcctaa ggctggccct ggtgtggtgc gaaagaaccc tggtgtgggc
      721 aacggagacg acgaggcagc tgagttgatg cagcaggtca acgtattgaa acttactgtt
      781 gaagacttgg agaaagagag ggatttctac ttcggaaagc tacggaacat tgaattgatt
      841 tgccaggaga acgaggggga aaacgaccct gtat
//
