LOCUS       sequence-67887-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Cortical dynein and asymmetric membrane elongation coordinately 
            position the spindle in anaphase.
  JOURNAL   Cell. 2013 Jul 18;154(2):391-402. doi: 10.1016/j.cell.2013.06.010.
  PUBMED    23870127
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.06"; journalName: "Cell"; date: "2013-07-18- 
            18"; volume: "154"; issue: "2"; pages: "391-402"
COMMENT     Sequence Label: pTK157_mCherry-4.1G-CTD
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(542..586)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(599..619)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctgcataaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
       61 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
      121 agttatctag atccggtgga tcccgggccc gcggttaatc ttccccttcc tcagccaact
      181 ctgtttcttt gtgtaccacc actcttgtga ccgacatgtc agggtgctgc tctctggctt
      241 ccctgatcgc ctgagccagt gcctggtcat gatcaatatc tccatctcct gtgatcacaa
      301 tgcgtttctc aattcttgtt tcagaaattc caccttttac agtcttggtg atgtgtgtgg
      361 ttgtcgttgt tgacacggac tcagatgtga tggtttgtgc ggtcagtaac gtgcccgaat
      421 caccaccagc cccgccatca atctgtggag actcatatgt gatggttttg gtctcagttt
      481 ggacaatggg gacttccttg gtggagattt ctgtcctctc gagactagta cctccacctc
      541 cgctgtccat gtgctggcgt tcgaatttag cagcagcggt ttctttgaat ttggcggcgc
      601 cctgaaaata aagattctct ctagagcggc cgccaccggt ggagctccac cgatcccctc
      661 cggacttgta cagctcgtcc atgccgccgg tggagtggcg gccctcggcg cgttcgtact
      721 gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc caacttgatg ttgac
//
