LOCUS       sequence-67889-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter cel-mir-240
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(422..446)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggctca tatgtcatga
      121 gctccgcctt tactattcta tccaccacct acatatatta cagtttcatt tgcacacggc
      181 ggcttgtcat tttcctgtct attgctattt cgttgttaga aactttttca aatcgaggat
      241 tttgagacta gaatgcttga attgtactag catactggcc cccaaatctt cgcttagaaa
      301 tacagttcac aattatttta atggtgagta aagtaagtaa agggatagtt cccgatcgaa
      361 tatcagttgg ggtatttaca aaaagacaaa attgtaatgc cctgaatgat gttcaaagct
      421 tacccagctt tcttgtacaa agtggttgat ctagagggcc cgcagtgggg tcagcttcta
      481 ccggggcggc gtcccggggt cttggaactg catgcagact gcctgcttgg gaaacatact
      541 tctttatatg cccatatgga cctgctaagc tatggaatgt aaagaagtat gtatctcagg
      601 ccgggacctc tctcgccgca ctgaggggca ctccacacca cgggggccgc cgcggccgac
      661 agccagccag gcggctgtgg tgggggtgag gctggggcgg caggctgcct gggcgttggt
      721 ggacggtcgt gtccccgcca gcattgttct gtcactttgc ctgtgtca
//
