LOCUS       sequence-67890-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter cel-let-7
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(510..534)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggctca gttacactta
      121 tttattagaa acctagattt agagactttt agaataactt tacttcctag aacacatctc
      181 cctttgaatt tatatgtcta atttaacaac aagtactaat ccatttttca ggcaagcagg
      241 cgattggtgg acggtctaca ctgtggatcc ggtgaggtag taggttgtat agtttggaat
      301 attaccaccg gtgaactatg caattttcta ccttaccgga gacagaactc ttcgaagctg
      361 cgtcgtcttg ctctcacaac tttcttttcg ttttcttcct cctcttactt tcacctcttt
      421 gtttttcttt attttgtgct atcaattcgg agtcgcaaga actgtattcg gtgatcccta
      481 ttgatattct tttgtactgt attcggagaa cccagctttc ttgtacaaag tggttgatct
      541 agagggcccg cagtggggtc agcttctacc ggggcggcgt cccggggtct tggaactgca
      601 tgcagactgc ctgcttggga aacatacttc tttatatgcc catatggacc tgctaagcta
      661 tggaatgtaa agaagtatgt atctcaggcc gggacctctc tcgccgcact gaggggcact
      721 ccacaccacg ggggccgccg cggccgacag ccagccaggc ggctgtggtg ggggtgaggc
      781 tgggg
//
