LOCUS       sequence-67891-e         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter cel-lin-4
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(471..495)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggctca tatgctacca
      121 caatcggtcg gactcatcac acttaccttt caaatatcta aactattcct gaatataata
      181 aatcttatag tttttagatt ctagacaatt tctagagttt tggttggttt atgagtttat
      241 gcttccggcc tgttccctga gacctcaagt gtgagtgtac tattgatgct tcacacctgg
      301 gctctccggg taccaggacg gtttgagcag atcttttttt ctgttttcac ggggtttttt
      361 ttcacactgg gagctagttc caataaaatg gtattcgggg aaaagggtca aacgattatt
      421 ttcagaagtc gactacaata gagttccact aacgccaact gatgaagctt acccagcttt
      481 cttgtacaaa gtggttgatc tagagggccc gcagtggggt cagcttctac cggggcggcg
      541 tcccggggtc ttggaactgc atgcagactg cctgcttggg aaacatactt ctttatatgc
      601 ccatatggac ctgctaagct atggaatgta aagaagtatg tatctcaggc cgggacctct
      661 ctcgccgcac tgaggggcac tccacaccac gggggccgcc gcggccgaca gccagccagg
      721 cggctgtggt gggggtga
//
