LOCUS       sequence-67892-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter cel-lsy-6
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(436..460)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             813..854
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(894..913)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggctca tatggcaatt
      121 gagcaaagca tctgcaggtg cctgatattt tacggcttcc gcccattacc gtagctgaaa
      181 gagtataaat agaagattca tcgttcaaat gtttaatgtt cgacttttca aataatgtat
      241 tcctactaat ttctgaaaaa taagccatca aatgcgtcta gtatcaaaat catgtaaaaa
      301 ctgtaaaaca gattttgtat gagacgcatt tcgatgacgg gcacaacaga gacaccgccc
      361 ataagacggt atcttttgat ggtaagaggt gtgaatctat aatacgactg tgtgatccat
      421 gtgtcggtta agcttaccca gctttcttgt acaaagtggt tgatctagag ggcccgcagt
      481 ggggtcagct tctaccgggg cggcgtcccg gggtcttgga actgcatgca gactgcctgc
      541 ttgggaaaca tacttcttta tatgcccata tggacctgct aagctatgga atgtaaagaa
      601 gtatgtatct caggccggga cctctctcgc cgcactgagg ggcactccac accacggggg
      661 ccgccgcggc cgacagccag ccaggcggct gtggtggggg tgaggctggg gcggcaggct
      721 gcctgggcgt tggtggacgg tcgtgtcccc gccagcattg ttctgtcact ttgcctgtgt
      781 cagcaggtgg aagtgctcac cagacattcg aaggtaagcc tatccctaac cctctcctcg
      841 gtctcgattc tacgcgtacc ggttagtaat gagtttaaac gggggaggct aactgaaaca
      901 cggaaggaga caataccgga
//
