LOCUS       sequence-67894-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Cortical dynein and asymmetric membrane elongation coordinately 
            position the spindle in anaphase.
  JOURNAL   Cell. 2013 Jul 18;154(2):391-402. doi: 10.1016/j.cell.2013.06.010.
  PUBMED    23870127
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.06"; journalName: "Cell"; date: "2013-07-18- 
            18"; volume: "154"; issue: "2"; pages: "391-402"
COMMENT     Sequence Label: pTK38_mCherry-LGN-C
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             295..315
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             328..372
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
       61 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
      121 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
      181 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
      241 ctgtacaagt ccggagggga tcggtggagc tccaccggtg gcggccgctc tagagagaat
      301 ctttattttc agggcgccgc caaattcaaa gaaaccgctg ctgctaaatt cgaacgccag
      361 cacatggaca gcggaggtgg aggtactagt ctcgagcgac ttaatctctc agaccttcaa
      421 atggttcttg gtctgagcta cagcacaaat aactccataa tgtctgaaaa tactgaaatt
      481 gatagcagtt tgaatggtgt acgccccaag ttgggacgcc ggcatagtat ggaaaatatg
      541 gaacttatga agttaacacc agaaaaggta cagaactgga acagtgaaat tcttgctaag
      601 caaaaacctc ttattgccaa accttctgca aagctactct ttgtcaacag actgaagggg
      661 aaaaaataca aaacgaattc ctccactaaa gttctccaag atgccagtaa ttctattgac
      721 caccgaattc caaattctca gaggaaaatc agtgcagata ctattggaga tgaagggttc
      781 tttgacttat taagccgatt tcaaagcaat aggatggatg atcagagatg ttgcttacaa
      841 gaaaagaact gccatacagc ttcaacaaca acttccactc cccctaaaat gatgctaaaa
      901 acatcatctg ttcctgtggt atcccccaac acgg
//
