LOCUS       sequence-67895-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  rescues the phenotypes caused by LGN RNAi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Cortical dynein and asymmetric membrane elongation coordinately 
            position the spindle in anaphase.
  JOURNAL   Cell. 2013 Jul 18;154(2):391-402. doi: 10.1016/j.cell.2013.06.010.
  PUBMED    23870127
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.06"; journalName: "Cell"; date: "2013-07-18- 
            18"; volume: "154"; issue: "2"; pages: "391-402"
COMMENT     Sequence Label: pTK47_mCherry-LGN-hardened
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             290..310
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             323..367
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
       61 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
      121 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
      181 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
      241 caagtccgga ggggatcggt ggagctccac cggtggcggc cgctctagag agaatcttta
      301 ttttcagggc gccgccaaat tcaaagaaac cgctgctgct aaattcgaac gccagcacat
      361 ggacagcgga ggtggaggta ctagtctcga gatgagagaa gaccattctt ttcatgttcg
      421 ttacagaatg gaagcttctt gcctagagct ggccttggaa ggggaacgtc tatgtaaatc
      481 aggagactgc cgcgctggcg tgtcattctt tgaagctgca gttcaagttg gaactgaaga
      541 cctaaaaaca cttagcgcta tttacagcca gttgggcaat gcttatttct atttgcatga
      601 ttatgccaaa gcattagaat atcaccatca tgatttaacc cttgcaagga ctattggaga
      661 ccagctgggg gaagcgaaag ctagtggtaa tctgggaaac accttaaaag ttcttgggaa
      721 ttttgacgaa gccatagttt gttgtcagcg acacctagat atttccagag agcttaatga
      781 caaggtggga gaagcaagag cactttacaa tcttgggaat gtgtatcatg ccaaagggaa
      841 aagttttggt tgccctg
//
